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ACUPUNCTURE & ELECTRO-THERAPEUTICS
The International Journal
Volume 30, Numbers 1/2
Acupuncture & Electro-Therapeutics Res.,
Int. J., Vol. 30, pp. 1-14, 2005
0360-1293/05 $20.00 + .00
Copyright © 2005 Cognizant Communication Corp.
Printed in the USA
Involvement of Taurine in Penicillin-Induced Epilepsy and Anti-convulsive Effects of Acupuncture: A Preliminary Report
Qing Li,1* M.S., Assistant
Professor of Neurobiology
Jing-Chun Guo,2,3* Ph.D. Candidate, Associate Professor of Neurobiology
Hong-Bing Jin,2,3 B.S., M.S. Candidate
Jie-Shi Cheng,2,3 M.D., Professor of Neurobiology and Deputy Director of Institute of Acupuncture Research
Ru Yan,2,3 Ph.D., Associate Professor of Neurobiology
1Dept. of Physiology, Ningxia Medical
School, Yingchuan 750000
2National Key Laboratory of Medical Neurobiology
3Institute of Acupuncture Research
(WHO Collaborating Center for Traditional Medicine), Shanghai Medical School of Fudan University (Former Shanghai Medical University), Shanghai 200032 P.R. China
The potential role of taurine on epilepsy and acupuncture anti-convulsion was addressed in the present study. Epilepsy was induced by micro-injection of penicillin into hippocampus of Wistar rats. Taurine was applied by intraperitioneal (i.p.) injection. Electro-acupuncture (EA) was perforce on acupoints of DU 20 "Bai Hui" and DU 16 "Feng Fu" along DU channel. Epileptic grades were evaluated by electro-encephalography (EEG) and behavior score. We featured the dose-response relationship between taurine-treated epilepsy and epilepsy-only subjects, detected the effect of exogenous taurine on epilepsy and acupuncture treatment, and investigated taurine transporter immuno-activity in hippocampus using immunohistochemistry. It was found that: 1), taurine had a significant antiepileptic effect as applied at i.p. 20 mg/kg, 40mg/kg, 80mg/kg, especially at 40mg/kg in the rat model of penicillin-induced seizure. Animals were improved by one to three Racine grades in behavior and in frequency and amplitude of EEG. 2), Exogenous taurine enhanced the anti-convulsive effect of EA. Both behavior and EEG were improved in taurine-treated rats. EA inhibited epilepsy. Exogenous taurine improved epilepsy in a synergistic manner to EA. 3), EA increased the concentration of taurine transporter in hippocampus by comparing EA-treated epilepsy with normal control and penicillin only, or EA-treated plus taurine-treated epilepsy with taurine treated only epilepsy and penicillin only. The resulting data suggested that taurine may play an inhibitory role against epilepsy as an inhibitory amino acid in the central nervous system and EA may inhibit epilepsy via upregulating the concentration of taurine transporter to increase the release of taurine.
Key Words: taurine, epilepsy, electroacupuncture, taurine transporter.
Localization of Cholecystokinin and Vasoactive Intestinal Peptide in Lower Biliary Tract in Cats Following Electroacupuncture on the Right Qimen (LR14) and Riyue (GB24): An Immunohistochemistry Study
Yung-Ling Kuo, M.D., Research staff
Institute of Chinese Medicine Science, China Medical College, Taichung, Taiwan, Republic of China.
Jen-Hwey Chiu, M.D., Ph.D., Professor
Institute of Traditional Medicine, School of Medicine, National Yang-Ming University; Taiwan, Republic of China; Division of General Surgery, Department of Surgery, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Jaung-Geng Lin, M.D., Ph.D., Professor
Institute of Chinese Medicine Science, China Medical College, Taichung, Taiwan, Republic of China
Chin-Ling Hsieh, M.D., Ph.D., Professor
Institute of Chinese Medicine Science, China Medial College, Taichung, Taiwan, Republic of China.
Chew-Wun Wu, M.D., Professor
Division of General Surgery, Department of Surgery, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Accumulating evidence has shown that control of the motility of the sphincter of Oddi (SO) involves a complex interaction between nerves, neurotransmitters and gastrointestinal hormones such as vasoactive intestinal peptide (VIP) and cholecystokinin (CCK). Our previous studies demonstrated that electroacupuncture (EA) modulated the SO motility in cats and rabbits through activation of non-adrenergic non-cholinergic (NANC) pathway. This study was designed to investigate the changes of neurotransmitters such as CCK and VIP in lower biliary tract in cats receiving EA stimulation. After cats were anesthetized with intramuscular injection of ketamine hydrochloride, they were prepared to conduct EA stimulation on right Qimen (LR14) and Riyue (GB24). The parameters of EA were 6 pulses/ 3 sec and 45 pulses/ 3sec alternatively in frequency, 102 mA in intensity and 20 min in stimulation duration. After the completeness of EA stimulation, visceral organs such as gallbladder, duodenum, and the sphincter of Oddi were removed and frozen for immunohistochemistry localization of CCK and VIP. The results showed that the distribution of CCK-labeled cells in duodenum, gallbladder and SO were more and distinct after EA than before EA stimulation. Whereas, the VIP-labeled cells were significantly more and distinct in duodenum and SO, but not in gall bladder. We concluded that EA regulates the biliary motility though increasing the distribution of CCK- and VIP-containing cells in duodenum and the sphincter of Oddi.
Key Words: Electroacupuncture; Sphincter of Oddi; Motility; Vasoactive Intestinal Peptide; Immunohistochemistry; Cholecystokinin
Correspondence: Dr. Jen-Hwey Chiu, Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, No. 155, Li-Nong Street, Sec. 2, Pei-tou, Taipei, 112, Taiwan; Tel: (886) 2-2875-7537, E-mail: email@example.com
Aging-related Changes in the Transcriptional Profile of Cerebrum in Senescence-accelerated Mouse (SAMP10) is Markedly Reduced by Acupuncture
Jianchun Yu, M.D., Associate Profesor
Tao Yu, M.D., Associate Professor
Jinxian Han, Professor
Gerontological Department, No.1 Teaching Hospital of Tianjin University of Traditional Chinese Medicine
Brain aging plays a pivotal role in senescence process and is related to cognition impairments and memory deficit and increases the risk of neurological disorders, but ideal therapeutic intervention has not been found. Here we report a dramatic effect of acupuncture, a powerful non-drug therapy way, on preventing changes in gene expression profiles with aging in senescence-accelerated mouse (SAMP10). We show that 48 genes in 588 genes examined display 2-fold or greater changes in gene expression with aging. However, we find that acupuncture can completely or partially prevent expression alterations of almost all these genes induced by aging. Our findings highlight a role of acupuncture as a potential intervention in anti-aging process and clinical therapy in future.
Key Words: Acupuncture; Aging, Expression array; Microarray; Cerebrum; SAMP10; Acupoint
Correspondence: Jingxian Han, Gerontological Department, No.1 Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China; Tel: 0086-22-27383189, Fax: 0086-22-2738318
Efficacy of Ear-Point Stimulation on Experimentally Induced Seizure
Jia Shu, M.D., Lecturer, Department of
Rong-Yu Liu, M.D., Lecturer, Department of Neurobiology
Xian-Fen Huang, M.D., Professor, Department of Neurobiology
Institute of Acupuncture Research, WHO Collaborating Center for Traditional Medicine, Shanghai Medical College of Fudan University (Formerly Shanghai Medical University), Shanghai, 200032, P.R. China
This study was to observe the effects of ear-point stimulation on electrocorticogram of sensorimotor cortex and behaviors of rats with penicillin-induced seizure. The model of epilepsy was by injecting penicillin into the hippocampus. One hour later, the lower ½ auricular lobules containing ear-points Pizhixia, Shenmen_Zeng and Nao, etc. as humans, or great auricular nerve of seizure rats, were treated twice with electrical stimulation (parameters of stimulation were as follows: electrical current intensity 0.14~0.2 mA, frequency about 80Hz, 30 min on and 30 min off). The outcome showed that rats appeared epileptic-like electrocorticogram and convulsion behaviors 5 min after injected penicillin. When they were subsequently given the ear-point or great auricular nerve electrical stimulation separately, these epileptic-like electrocorticogram and seizure behaviors were definitely improved. These anti-seizure effects could be enhanced with hour extension of electrical stimulation. If the great auricular nerve of seizure rat was severed before electrical stimulation ear-points, the effects of anti-seizure disappeared. Otherwise, the seizure rats given sham ear-point electrical stimulation (the experimental conditions were same as that of ear-point stimulation other than electric current being no applied) did not show any improvement for epileptic-like electrocorticogram and seizure behaviors. Based on the results above, it was suggested that ear-point electrical stimulation could cause certainly efficacy of anti-seizure, which may be relative with the great auricular nerve.
Key Words: Electrical Stimulation; Ear-Point; Great Auricular Nerve; Penicillin-Induced Seizure; Electrocorticogram; Anti-Convulsion; Rat
Correspondence: Xian-Fen Huang, M.D., Department of Neurobiology, Shanghai Medical College of Fudan University, 13 Yi Xue Yuan Road, Shanghai, 200032, P.R. China, Tel: 86-21-54237611. Email: firstname.lastname@example.org
The Future of the Application of the Bi-Digital O-Ring Test in Sports Psychology
Kemal Nuri Ozerkan, M.D., F.I.C.A.E.
Assistant Professor of Department of Sports and Health Sciences, Istanbul University, School of Physical Education and Sport, Avcilar 34850, Istanbul, Turkey.
The Bi-Digital O-Ring Test, originally developed by Dr. Omura, utilizes changes in the degree of strength of voluntary movements of muscles of the fingers under a definite muscle tonus, making Bi-Digital O-Rings, as an indicator of pathology in the body. Research in Sports Psychology can use the classical measurement methods and Bi-Digital O-Ring Test method comparatively and thus produce new findings regarding the reliability and certainty of the Bi-Digital O-Ring Test test. It seems probable that by using the non-invasive Bi-Digital O-Ring Test test, it is possible to measure enzymes, hormones and neuro-transmitters instantaneously and assess a sports person's actual psychological and physiological performance, and thereby help them reach their peak performance levels during both exercise and competitions.
Keywords: Sports Medicine; Sports Psychology; Bi-Digital O-Ring Test; Cephalic Hypertension Syndrome; Nucleus Reticularis Giganto-Cellularis; Raphe nuclei; Serotonin; Repression
Correspondence: Tel: +90 212-233 36 36; Fax: +90 212-233 52 81; Email: email@example.com
Excessive Use of Steroid Hormone & Beneficial Effects of True ST. 36 Acupuncture on Malignant Brain Tumors - Part I; How to Estimate Non-Invasively Presence of Excess Dose of Steroid Hormone in Patients, Baseball Players & Other Professional Athletes from its Toxic Effects on Heart & Pancreas, as well as Persistent or Recurrent Infection - Part II
Yoshiaki Omura, MD, ScD, FACA, FICAE, FAAIM,
Director of Medical Research, Heart Disease, Research Foundation; Adjunct Prof. Dept. of Community & Preventive Medicine, New York Medical College; President, Int'l College of Acupuncture & Electro-Therapeutics; Prof., Dept. of Non-Orthodox Medicine, Ukrainian National Keiv Medical University
Using accurate organ representation areas map of the face, originally mapped by the author using Bi-Digital O-Ring Test Resonance Phenomena between two identical substances, one can make quick non-invasive screening of diseases by visual inspection, particularly if it is chronic degenerative disease, as they often develop deep crease or creases or discoloration on the pathological organ representation area. However, even if there are no visible abnormalities in the organ representation areas, the author found that when the individual is using excessive Steroid Hormones for malignant brain tumors, other medical purposes, and competitive sports, not only did the left ventricle and pancreas become very abnormal when examined by the Bi-Digital O-Ring Test, and Steroid Hormone accumulate in these organs with abnormally increased 8-OH-dG & TXB2, and Folic Acid & Telomere markedly reduced, but also the organ representation areas of the pancreas and left ventricle on the face showed similar abnormalities. Thus, using the Bi-Digital O-Ring Test, one can quickly and non-invasively screen the Steroid Hormone induced abnormalities of the heart and pancreas, and their organ representation areas of various parts of the body, including the face, tongue, ears, hands and feet. For malignant tumors including brain tumors, acupuncture on True ST. 36 or St. 37 was found to be highly beneficial by reducing cancer cell telomere to practically 0, while increasing normal cell telomere moderately. The author's study over the past 15 years indicates that photographs of the human body, including pictures that appear in newspapers and magazines, have almost identical information as the information taken directly from the body surfaces of patients or individual athletes. Some examples of the application of this principle for the non-invasive estimation of the presence of Steroid Hormones using a photograph of the individual receiving the Steroid Hormone for medical reason, or for the purpose of gaining an advantage in competitive sports, are shown in this article. While excessive Steroid Hormone use damages the heart and pancreas and hides symptoms of infection, additional use of Vitamin C also inhibits antibiotics and antiviral agents and cannot control sever infection, as suspected in the cases of late Pope John Paul II and late Palestinian leader Yasser Arafat.
Key Words: Steroid Hormone; Decadron; Heart and Pancreas; Baseball Players; Muscle Builders; Competitive Athletics; Malignant Brain Tumors (Anaplastic Astrocytoma and Glioblastoma Multiforme); Hyperglycemia
Correspondence: Tel: (212) 781-6262; Fax: (212) 923-2279