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The International Journal

Volume 30, Numbers 3/4

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 30, pp. 185-199, 2005
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Electroacupuncture Enhances Striatal Neurogenesis in Adult Rat Brains After a Transient Cerebral Middle Artery Occlusion

Zeng-Jin Yang,* Ph.D. Postgraduate Student
Di-Han Shen,* Ph.D. Postgraduate Student
Xin Guo, M.S. Postgraduate Student
Feng-Yan Sun, Ph.D., Professor of Neurobiology

State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, Shanghai 200032, P.R. China

In this study, we investigate the effects of electroacupuncture (EA) on ischemia-induced neurogenesis in the striatum of adult rat brains with a 30-minute middle cerebral artery occlusion. Injection of bromodeoxyuridine (BrdU, 30 mg/kg, i.p., cell proliferation marker) and 1, 1'-dioctadecyl-6, 6'-di(4-sulfophenyl)-3, 3', 3', 3'-tetramethylindocarbo-cyanine (DiI, 1 ug/ ul, i.c.v, lipophilic neuronal tracer) combined with multiple fluorescence immunostaining were used to determine whether the proliferated cells were newly generated neurons and where they originated from in the brain. We demonstrated that EA treatment (60 Hz 1 s and 2 Hz 3 s alternately at an intensity of 10 mA for 20 min on "Fengfu", GV.16 and "Jinsuo", GV.8) enhanced stroke-induced striatal neurogenesis in rat brains as follows: 1) EA increased the number of BrdU+ cells,  indicating that it activiates cell proliferation; 2) EA increased BrdU+/CRMP-4+ (collapsing response mediated protein-4, immature neuron marker) and BrdU+/MAP-2+ (microtubule-associated protein 2, mature neuron marker) cells, suggesting that it facilitates neurogenesis and maturation of newly generated neurons; 3) EA expanded the distribution of DiI-stained cells in the striatum. Moreover, most BrdU+/CRMP-4+ or BrdU+/MAP-2+ cells in the striatum were observed DiI+ staining. Thus, the results suggest that striatal newborn neurons mainly migrate from the cells lining ventricle. Therefore, we conclude that EA can improve neuronal regeneration, newborn neuron migration and their maturation in the striatum of adult ran brains after stroke.

Key Words: Electroacupuncture; Neuronal regeneration; Striatum; Stroke; Neurogenesis; Migration

Correspondence: Feng-Yan SUN, State Key Laboratory of Medical Neurobiology, Shanghai Medical College, Fudan University, P.R. China. Tel: +86-21-54237774; E-mail: fysun@shmu.edu.cn

*These authors contributed equally to this article.

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 30, pp. 205-206, 2005
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An Exploratory Study of the Relationship Between the Midday-Midnight Law and Electrical Conduction Properties of Corresponding Acupuncture Points

Myeong Soo lee, Ph.D., Senior Research Fellow
Center for Integrative Medicine, Institute of Medial Science, Wonkwang University, Korea.
Present address: Complementary Medicine, Peninsula Medical School, Universities of Exeter & Plymouth, UK (drmslee@gmail.com)

Byung-Cheul Shin, OMD, Ph.D., Professor of Oriental Rehabilitation
Oriental Rehabilitation, College of Oriental Medicine, Wonkwang University, Korea

Dong-Myong Jeong, Ph.D., Professor of Electronic Engineering
Institute of Biomedical Engineering Research, Wonkwang University, Korea

The midday-midnight law is a well-known empirical law in Oriental medicine stating that the circadian rhythm of internal organs influences their paired organs according to the time of day. However, there has been little research to test the purported relationship between the time of day and organ function. The purpose of this preliminary study was to investigate whether the midday-midnight law applies to the meridian activity and the electrical conduction properties of the acupuncture points. We used the digital readout instrument (MERIDIAN) to measure electrical conduction properties at 12 acupuncture points corresponding to the 12 meridians at four times: 12:00, 14:00, 22:00, and 24:00 hours. There were no significant differences in the electrical current values measured at the four times in any meridian. However, the mean current values of 12 acupuncture points changed significantly over time (P < 0.0001). the mean current values were significantly higher at 14:00 (P < 0.001), 22:00 and 24:00 hours (P < 0.001) than at 12:00 hours. The highest current value occurred at 22:00 hours and was significantly higher than 24:00 hours (P < 0.05). Although these do not conclusively support the tradition midday-midnight theory, our data suggest the existence of some type of daily variability in electrical current.

Key words: Acupuncture points; Circadian rhythm; Electrical conduction of acupuncture point; Meridian

Correspondence: D.M. Jeong (dmz@wonkwang.ac.kr), Wonkwang University, Iksan 570-749, South Korea. Tel: 82-63-858-6741; Fax: 82-63-858-6742

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 30, pp. 207-217, 2005
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Electro-acupuncture Improves Epileptic Seizures Induced by Kainic Acid in Taurine-depletion Rats

Hong-Bing Jin,1 B.S., M.S. Candidate
Bing Li,1,2,3 B.S., M.S. Candidate
Jing Gu,1 B.S.
Jie-Shi Cheng,1,2 M.D., Professor of Neurobiology & Deputy Director of Institute of Acupuncture Research
Ru Yang,1,2 Ph.D., Associate Professor of Neurobiology

1National Key Laboratory of Medical Neurobiology,
2Institute of Acupuncture Research (WHO Collaborating Center for Traditional Medicine) Shanghai Medical College of Fudan University (Former Shanghai Medical University) Shanghai 200032, P.R. China
3Dept. of Physiology, Guangxi Medical University
Electro-acupuncture (EA) partially inhibited epilepsy with great success. The biological basis underlying EA anti-convulsion remained uncertain, which resulted in limited application and slow improvement of acupuncture. Our previous study indicated that taurine may play an inhibitory role against epilepsy as an inhibitory amino acid in the central nervous system and EA may inhibit epilepsy via up-regulating the expression of taurine transporter to increase the release of taurine. Involvement of taurine in kainic acid (KA)-induced epilepsy and anti-convulsion of EA was further addressed on taurine deficiency animal in the present work. We instituted endogenous taurine-deficiency model by supplementation of beta-alanine (3%) in drinking water for continuous 10 days initially, injected KA into lateral cerebral ventricle to induce epileptic seizure, and performed EA treatment on DU26 "RenZhong" and K1 "YongQuan" acupoints by an EA apparatus (Model G6805-2) using successive wabes with the frequency 64Hz and the current intensity 0.8-1.0mA for 30 minutes in Sprague-Dawley (SD) rats. Taurine levels markedly decreased in cortex, hippocampus, striatum and cerebellum of rats after beta-alanine administration by fluore-HPLC measurement. EA alleviated epileptic activity in rats at 3.5h time point after KA injection, whereas beta-alanine-induced taurine depletion rendered rats more susceptible to KA-induced epilepsy. Taurine transporter level increased after EA treatment. These results suggested that taurine participated in epileptogenesis and EA may be related to taurine in controlling epileptic seizure.

Key words: Taurine depletion; Epilepsy; Electro-acupuncture; Taurine transporter; Kainic acid; Rat

Correspondence: Ru Yang. Institute of Acupuncture Research Shanghai Medical College of Fudan University, Shanghai 200032, P.R. China. Tel: 86-21-54237231; E-mail: ruyang@shmu.edu.cn

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 30, pp. 219-261, 2005
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Beneficial Effects & Side Effects of DHEA: True Anti-Aging & Age-Promoting Effects as Well as Anti-Cancer & Cancer-Promoting Effects of DHEA Evaluated From the Effects on the Normal & Cancer Cell Telomeres & Other Paramters


Director of Medical Research, Heart Disease Research Foundation; Adjunct Prof. Dept. of Community & Preventive Medicine, New York Medical College; President, Int'l College of Acupuncture & Electro-Therapeutics; Prof., Dept. of Non-Orthodox Medicine, Ukrainian National Kiev Medical University
The author evaluated the effects of DHEA (Dehydroepiandrosterone) on the amount of telomeres of normal cells & cancer cells and found the following: Contrary to literature, which often recommended 25-50 mg of DHEA daily for the average adult human being, the author found that, depending on the individual, the maximum increase of normal cell telomere was obtained by a single optimal dose of 1.25-12.5 mg. This was examined in 50 people, both males and females, between the ages of 20-80 years old. When one optimal dose was given to each individual, the average telomere amount in normal tissues, measure in Bi-Digital O-Ring Test units, often increased from anywhere between 25-300 ng to between 500-530 ng. Cancer cell telomere reduced from higher than 1100 ng to less than 1 yg (=10-24) with equally significant normalization of abnormal cancer parameters (such as Integrin a5b1, Oncogen C-fosAb2, Acetylcholine, etc.) Circulatory improvement and an increase in grasping force of up to 25% were also detected, along with the changing of a few white hairs to black hairs. The beneficial effects of one optimal dose of DHEA generally lasted between 1 to 4 months, though in some individuals it lasted for a much shorter period of time due to a number of negative factors such as excessive stress/work, excessive exposure to low temperatures and toxic substances, or use of common pain medicines. On the other hand, if a patient took an excessive does of DHEA, the amount of normal cell telomere decreased, while there was an increase in cancer cell telomere. It was found that those who took an overdose of 25-50 mg daily for more than 3 months had a high incidence of cancer of the prostate gland, breast, colon, lung, and stomach. Also, when the average normal cell telomere levels were less than 110 ng, compared with a normal value of 120-130 ng, and when DHEA in different parts of the body was also extremely low (less than 1-2 ng), one could suspect the possible presence of a malignant tumor somewhere in the body. When normal cell telomere was less than 110 ng, most individuals felt very weary with marked tiredness in the eyes, and grasping force was often reduced.

Key words: DHEA; Anti-aging effect of DHEA; Age-promoting effect of DHEA; Anti-cancer effect of DHEA; Cancer-promoting effect of DHEA; Bi-Digital O-Ring Test; Pain; Circulatory disturbance; White & black hairs; Prostate cancer; Astrocytoma; Non-steroidal Anti-inflammatory drugs (NSAIDs); Hand writings

Correspondence: Yoshiaki Omura, MD, ScD, 800 Riverside Dr. (8-I) New York, NY 10032. Tel: (212) 781-6262; Fax: (212) 923-2279

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 30, pp. 263-273, 2005
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Effects of Acupuncture on nNOS and iNOS Expression in the Rostral Ventrolateral Medulla of Stress-induced Hypertensive Rats

Yu-Ling Huang, M.D. Postgraduate Student
Ming-Xin Fan, Ph.D. Postgraduate Student
Jin Wang, Ph.D., lecturer of Physiology
Li Li, M.D., Associate Professor of Physiology
Ning Lu, M.D., Associate Professor of Physiology
Yin-Xiang Cao, M.D., Associate Professor of Physiology
Lin-Lin Shen, Ph.D., M.D., Professor of Physiology
Da-Nian Zhu, Ph.D., M.D., Professor of Physiology

Department of Physiology and Pathophysiology, Shanghai Medical College of Fudan University, Shanghai 200032, China

This study was to observe the changes of the neuronal and inducible nitric oxide synthase (nNOS & iNOS) as well as their mRNAs in the rostral ventrolateral medulla (RVLM) of stress-induced hypertensive rats before and after acupuncture, and thereby to infer the curative mechanism of acupuncture on hypertension. The result indicated that the systolic blood pressure (SBP) of stress group rats was increased significantly (P < 0.01), and the mRNA (P < 0.01) were obviously elevated, while those of iNOS (P < 0.05, P < 0.01 and P < 0.01) were evidently lowered in the stress-induced hypertensive rats. Electroacupuncture (EA) points at "Zunsanli" (St. 36) and "Lanwei" (Extra 37) on the same hindlimb were stimulated by an EA apparatus (Type G6802-2) with dense sparse wave (4-20Hz) and 4mA intensity. EA application could return the SBP (P < 0.05), and the changes on the expression of both nNOS and iNOS (P < 0.05, P < 0.01 and P < 0.01). These results suggest that he curative mechanism of acupuncture on stress-induced hypertension is related to the changes of nNOS and iNOS in the RVLM of rats.

Key words: Rostral ventrolateral medulla; Neuronal nitric oxide synthase; Inducible nitric oxide synthase; Stress-induced hypertension; Acupuncture

Correspondence: Da-Nian Zhu. Ph.D., Dept. of Physiology and Pathophysiology, Shanghai Medical College of Fudan University, Shanghai 200032, P.R. China. Tel: 86-21-54327405; E-mail: dnzhu@shmu.edu.cn

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 30, pp. 275-288, 2005
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Evaluation of Electroacupuncture on Ovariectomized Rats: Implications of Modern Scientific Mechanisms on Acupuncture Curing Woman Perimenopausal Syndrome

Hong Zhao Ph.D., Lecturer of Neurobiology and Integrative Medicine
Bo-Ying Chen, M.D., Professor of Neurobiology and Integrative Medicine

Department of Neurobiology and Integrative Medicine, Institute of Acupuncture Research (WHO Collaborating Center for Traditional Medicine), Shanghai Medical College of Fudan University, (The former Shanghai Medical University) 138 Yi-Xue-Yuan Road, Shanghai 200032, Shanghai. China.

Acupuncture has been used for treatment of female reproductive disorders dated back at least 2000 years. It has been known to promote homeostasis, modulated hormonal disturbance and induce ovulation. While the increasing popularity of acupuncture therapy demands a serious debate about its scientific documentation. This article is intended to present the experimental data about the effects of electroacupuncture at Guanyuan (RN 4), bilateral Zhongji (RN 3), Sanyinjiao (SP 6) and bilateral Zigong (EXCA 1) in the ovariectomized rats. The EA stimulation produced from a Model G6805-H EA apparatus, and the stimulation parameters were frequently of 3 Hz and intensity about 1-2mA, which was progressed in recent years by our group, so as to provide preliminary scientific background of acupuncture therapy of female perimenopausal syndrome.

Key words: Electroacupuncture; Ovariectomized rats; Perimenopausal syndrome

Correspondence: Professor Bo-Ying Chen. Tel: 86-21-54237693; E-mail: chen_bo_ying@hotmail.com