ognizant Communication Corporation

The International Journal

Volume 32, Numbers 3/4

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 32, pp. 179-193, 2007
0360-1293/07 $20.00 + .00
Copyright © 2007 Cognizant Communication Corp.
Printed in the USA

Neuronal Specificity of Needling Acupoints at Same Meridian: A Control Functional Magnetic Resonance Imaging Study with Electroacupuncture

Jun-hai Zhang,1 Xiao-ding Cao,2 Ji Li,3 Wei-jun Tang,1 Han-qiu Liu,1 Xiao-yuan Feng1

1Department of Radiology, Huashan Hospital; Department of Medical Imagning, Shanghai Medical College, Fudan University, Shanghai, China
2Department of Neurobiology, Shanghai Medical College, Fudan University, Shanghai, China
3Department of Chinese Traditional Medicine, Huashan Hospital, Shanghai, China

Abstract: The purpose of this study was to investigate the neuronal specificity of needling acupoints at same meridian by functional Magnetic Resonance Imaging (fMRI). The selected acupoints GB34 (Yanglinquan ) and GB39 (Xuanzhong) were at the same gallbladder meridian based on traditional Chinese medicine. In our study we devise three distinct EA (electroacupuncture) manipulations: real EA (deep needling at acupoints), sham EA (deep needling at no-meridian points) and shallow EA (subcutaneous needling at acupoints). Twelve healthy volunteers with right-handiness were enrolled and received three different EA manipulations in counter-balanced orders. DeQi scores were used to evaluate the degree of needling sensation. We found real EA can induce significant stronger needling sensation than sham EA and shallow EA. Multisubjects group mean analysis showed that pain-related cortex including primary and secondary somatosensory cortex (SI and S II), anterior cingulated cortex (ACC), insula were involved in three EA simulation. Bilateral activation of prefrontal gyrus and occipital cortex were exclusively found in real EA. Deactivation over the rostral segment of ACC was also shown in real and shallow EA. Further paired two difference analysis indicated that rea EA induced higher activation than sham EA over bilateral prefrontal gyrus, right-side occipital gyrus and deactivation over the rostral segment of ACC. I the comparing with real EA versus shallow EA, there was right-side activation over the SI, SII, motor cortex, ACC, insula, thalamus, hippocampus, occipital cortex, and cerebellum; also activation over bilateral prefrontal gyrus, caudate and pons. Although no signification activation was found over periaqueductal gray (PAG), further analysis showed the mean and maximal signal changes were different under three EA manipulations. We concluded that EA at analgesic acupoints of the same meridian maybe involved the pain-related neuromatrix especially the hypothalamus-limbic system; deep EA at meridian points could elicit stronger needling sensation and modulate the pain-related neuromatrix more effectively than EA at nonmeridian points or shallow EA at meridian points.

Key Words: functional MRI; Electroacupunture, Meridian; Brain mapping

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 32, pp. 195-210, 2007
0360-1293/07 $20.00 + .00
Copyright © 2007 Cognizant Communication Corp.
Printed in the USA

Immunohistochemical and Electron Microscopic Study of the Meridian-like System on the Surface of Internal Organs of Rats

Su Hong, Ph.D. Candidate
Jung Sun Yoo, Ph.D. Candidate
Ju Young Hong, Ph.D. Candidate
Byung-Cheon Lee, Ph.D., Senior Researcher
Kwang-Sup Soh, Ph.D., Professor of Physics
Biomedical Physics Laboratory, School of Physics, Seoul National University, Seoul, Korea

Sang-Hee Lee, Ph.D.
Young-Joong Kim, Ph.D.
High-voltage electron microscopy Team, Korea Basic Science Institute, Daejeon, Korea

Dae-In Kang, O.M.D.
Byung Soo Ahn, O.M.D.
Korean Institute of Herbal Acupuncture, Seoul, 1570200, Korea

Hee-Jong Woo, Ph.D., V.M.D., Professor of Veterinary Medicine
Laboratory of immunology, College of Veterinary Medicine, Seoul National University, Seoul, Korea

Abstract:Threadlike structures on the surfaces of mammalian internal organs were recently observed, and they can be the anatomical basis of the meridian-like network of internal organs as detected by the Bi-Digital O-Ring test imaging method. Morphological and histological studies were performed to reveal their novel features by using confocal and electron microscopy, and there is evidence that reveals that they have a ductal structure in which some liquid flows. They form a part of the network of acupuncture meridians that is thought to be a circulatory system distributed throughout an animal body. In order to confirm further the circulatory function it was necessary to provide evidences for the existence of endothelial cells and the inner boundaries of the microchannels that from the threadlike ducts. In this work we performed immunohistochemical studies for the identification of endothelial cells. In addition, we identified the extracellular matrices between the microchannels in the threadlike structure by using anti-fibronectin, elastin, laminin, and collagen type IV. Since immunohistochemical data are not sufficient for definite proof we supplemented the analysis with ultra structural information by suing transmission electron microscopy (TEM). Yet TEM images show only ultrathin sections (50 nm) of a specimen, we needed to take 3-dimentional images to provide a visual demonstration of the microchannels in the threadlike ducts. We conducted high voltage electron microscopic studies for this purpose and obtained corroborating tomograms that exhibit the microchannels. In conclusion, the meridian-like tissues are ducts with microchannels, which implies the circulatory function of the acupuncture meridian network.

Key Words: Acupuncture; Meridian-like network; Bi-Digital O-Ring test; Bonghan duct; Immunohistochemistry; Transmission electron microscopy; High voltage electron microscopy.

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 32, pp. 211-221, 2007
0360-1293/07 $20.00 + .00
Copyright © 2007 Cognizant Communication Corp.
Printed in the USA

The Fundamental Frequency of Voice and its Correlations with the Five Sounds of the Traditional Chinese Medicine

Fernando Cesar Dotta de Barros, M.D., M.Sc.
Otolaryngologist; Acupuncutrist; Medical Associate at the Sector of Acupuncture at the Universidade Federal de São Paulo-UNIFESP

Soraia Elias de Barros, B. Sc.
Speech and Hearing Disorders Therapist, Director of Oto-Rhino Clinic at São Paulo, Brazil

Abstract:The Fundamental Frequency of Voice (F0) is one of the patterns of voice evaluation, where the sound of a prolonged vowel spoken is measured by a chromatic tuner, and the result is obtained in a musical tone. We investigated the correlations of the Fundamental Frequency of Voice and the 5 sounds of Traditional Chinese Medicine. We determined the tones corresponding to the sounds related in the ancient book "Ling Shu," which are Zhi, Yu, Gong, Shang, Jue, using the Bi-Digital O-Ring Test and histological slides of the following human organs: heart, kidney, pancreas, lung and liver. We also investigated the F0 in interned patients with the following western medicine diagnosis: schizophrenia, kidney failure and hepatic failure. The results are discussed, and suggest that the F0 can be used as a complementary diagnostic tool in Chinese Medicine and Acupuncture.

Key Words: voice analysis, fundamental frequency, Chinese Medicine, Five Musical Tones, musical scale, Bi-Digital O-Ring test, schizophrenia, kidney failure, hepatic failure.

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 32, pp. 223-233, 2007
0360-1293/07 $20.00 + .00
Copyright © 2007 Cognizant Communication Corp.
Printed in the USA

Treatment of Intractable Dry Eyes: Tear Secretion Increase and Morphological Changes of the Lacrimal Gland of Rabbit After Acupuncture

Lan Gong Ph.D.
Assistant Prof. Dept. of Ophthalmology, EYE & ENT Hospital of Fudan University, Shanghai, China.

Xinghuai Sun Ph.D.
Prof. & Chairmen, Dept. of Ophthalmology, EYE & ENT Hospital of Fudan University, Shanghai, China.

Abstract:Research on the effect of acupuncture for tear secretion and morphological changes of the lacrimal gland. Acupuncture therapy was given on the rabbits of New Zealand origin. The needles were inserted into the following acupoints around the right eye: Extra 1 (Taiyang), BL 2 (Zanzhu) and SJ 23 (Sizhukong). The Schirmer Test I (S1T, The Schirmer test is probably the most commonly performed method of measuring aqueous tear production) values pre and post acupuncture therapy were blindly recorded. The lacrimal glands of the normal lacrimal gland group were stained by Hematoxylin and Eosin (HE) for light microscope examination and observed by transmission electron microscope. The S1T value was about sixty percent higher after acupuncture than before. Meanwhile changes also took place in the morphology of lacrimal gland tissue, indicated an active glandular function of synthesis and secretion. Acupuncture can increase lacrimal secretion by stimulating the rabbit lacrimal glandular function of synthesis and secretion.

Key Words: Acupuncture; Schirmer Test I (S1T); Lacrimal Gland; Morphology; Transmission Electron Microscopy; Dry Eyes

Acupuncture & Electro-Therapeutics Res., Int. J., Vol. 32, pp. 235-269, 2007
0360-1293/07 $20.00 + .00
Copyright © 2007 Cognizant Communication Corp.
Printed in the USA

Rapid Screening and Diagnosis of Various Cancers From Human Voice Using Bi-Digital O-Ring Test Resonance Phenomenon Between 2 Identical Substances i.e. Between Microscope Slide of Specific Cancer Tissue & Cancer Information in the Sound of Human Voice, and Detection of Myocardial Damage & Infection from Human Voice

Yoshiaki Omura, MD, ScD, FICAE
Director of Medical Research, Hear Disease, Research Foundation; Adjucnt Prof. Dept. of Community & Preventive Medicine, New York Medical College; President, Int'l College of Acupuncture & Electro-Therapeutics; Prof., Dept. of Non-Orthodox Medicine, Ukrainian National Kiev Medical University

Abstract:Since 1982, the author has been successfully using Bi-Digital O-Ring Test (BDORT) electro-magnetic resonance phenomenon between 2 identical substances; i.e. between specific cancer (in vivo) of a patient and a microscope slide of identical cancer tissue to detect cancers at a very early stage. Since 2000, the author has found that when BDORT is performed while an individual with a malignant tumor is talking, BDORT became (-1 ~ -10) in 94% of 200 cases and the number of openings (-) is approximately proportional to the degree of abnormality up to -10. Thus, cancer can be screened from voice within 1 min., without any instruments. Also, when cancer-free persons hold slides of cancer tissue or 10ng or higher Oncogene C-fos Ab2 or Integrin a5b1 while talking over a short or long distance, BDORT always opens. To identify the type of the patient's malignancy, if BDORT is performed by an examiner holding a microscope slide of cancer tissue identical to the patient's cancer, characteristic strong BDORT resonance phenomenon appears with opening of all O-Rings formed between thumb and other fingers. When patients with myocardial infarct or various infections speak, no O-Rings open without holding identical pathological substances. Thus, it is possible to detect any type of malignancy from the voice, as long as a set of about 35 different tumor tissue slides is available. Based on these new findings, the author concluded that the voice of an individual with a malignancy carries information about the amount and structure of molecules present in malignant tissue.

Key Words: Non-Invasive Diagnosis; Cancer; Alzheimer's Disease; Asbestos; Bi-Digital O-Ring Test; Human Voice; Integrin a5b1; Oncogene C-fos Ab2; Myocardial Infarct; Cardiac Troponin I; Infection; Hand Writing Analysis.