Acupuncture & Electro-Therapeutics Research 39(3-4) Abstracts

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Integrative Approach to Diagnosis of Genital Human Papillomaviruses (HPV) Infection of Female

Momir Dunjic, MD, PhD, F.I.C.A.E., Cert. ORT-MD (4 Dan), Associate Prof. of Ob/Gyn at School of Medicine, University of Pristina; Asoc. Prof of Complementary Medicine at Pharmaceutical Faculty, European University Belgrade; University Alma Mater Europea - ECM; President Serbian Association of Integrative Medicine; President Acupuncture and Quantum Medicine Section; Serbian Medical Society; Member of Board European Society of Integrative Medicine (ESIM-Berlin), Serbia

Slavisa Stanisic, MD, PhD, F.I.C.A.E., Cert. ORT-MD (2 Dan), Prof. of Ob/Gyn at School of Medicine, University of Pristina; Pharmaceutical Faculty European University Belgrade; University Alma Mater Europea-ECM; Vicepresident Serbian Association of Integrative Medicine; Vicepresident Serbian Ob/Gyn Associtaon; Member of Board European Society of Integrative Medicine (ESIM-Berlin), Serbia

Dejan Krstic, PhD, Associate Prof. Faculty of Occupational Safety, University of Nis; President of Section for Nonionizing radiation, Serbian Association of Integrative Medicine, Serbia

Miodrag Stanisic, MD, Cert. ORT-MD (1 Dan), Clinic of Surgery, University Clinical Center Pristina; General secretary Serbian Association of Integrative Medicine, Serbia

Z. Jovanovic Ignjatic, MD, Cert. ORT-MD (1 Dan), Physical and Quantum Medicine, Acupuncture and Homeopathy private practice Cert. ORT-MD (1 Dan), Belgrade; Vicepresident Acupuncture and Quantum Medicine Section, Serbian Medical Society; Vicepresident Serbian Association of Integrative Medicine, Serbia

Marija Dunjic, student, School of Medicine, University of Belgrade, Serbia

(Correspodence: Momir Dunjic, Medicinski fakultet,Univerziteta u Pristini, Anri Dinane bb, 38220 Kosovska Mitrovica, Srbija; mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it , Phone: +381 62 222555) (Received: June 25th, 2013; Accepted with Revisions: January 20th, 2013)

ABSTRACT:

Human papillomavirus (HPV) is a virus from the papillomavirus family that is capable of infecting humans. Some types of HPVs cause warts, while others can lead to cancers of the cervix, vulva, vagina, penis, oropharynx and anus. Highrisk human papillomavirus (hr HPV) has been detected in almost all cervical squamous cell carcinomas and adenocarcinomas. All patients examined by colposcopy. Cervical swab is routinely done and patients are screened with both HPV DNA by Real Time Polimerase Chain Reaction (RT PCR) testing and Pap testing. Pictures obtained by colposcopy were examined by indirect Bi-Digital O-Ring Test (BDORT) by using reference control substance (RCS): HPV 16, HPV 18, and Integrin α5 β1. BDORT was developed by Prof. Omura Y. of New York and received U.S. patent in 1993. For detection of HPV DNA we used RT PCR and standard Qiagen method which detect 18 types (16,18,31,33,35,39,45,51,52,56,58,59,68, 6,11,42,43,44) of HPV from smear. From 63 patients where is BDORT indicated presence of HPV, in 49 patients (77,8%) RT PCR confirmed presence of HPV. From 63 patients in 54 patients (85,7%), we detected, by colposcopic exam, some kind of lesions associated with HPV infection. Results obtained by RT PCR: one type (1/18) of DNA HPV in 25 patients (51,02%), 2 types (2/18) in 15 patients (30,61%) and 3 types (3/18) in 9 patients (18,37%). Although BDORT results usually have higher sensitivity and detection rate is much higher, it can be used together with RT PCR in detection of HPV and cervical lesions associated with HPV infection.

3,5,11 Needles: Looking For The Perfect Number of Needles--A Randomized And Controlled Study

Francesco Ceccherelli, M.D., F.I.C.A.E. Former Research Professor of Anesthesiology and Intensive Care, Department of Pharmacology and Anesthesiology, University of Padova; A.I.R.A.S. Padova, Italy

Elena Marino M.D. Pain Therapy Unit, ASL Salerno Unica, Salerno

Antonio Caliendo M.D. Pain Therapy Unit, ASL Salerno Unica, Salerno

Rossana Dezzoni, M.D. Department of Perioperative Medicine, Intensive Care and Emergency, Cattinara University Hospital, Trieste, Italy.

Antonella Roveri, M.D. Department of Biologica Chemistry, University of Padova, A.I.R.A.S. – Padova. Italy

Giuseppe Gagliardi, M.D., Ph.D., F.I.C.A.E. Head of Intensive Care Unit of ULSS 16, S.Antonio Hospital, A.I.R.A.S. – Padova, Italy

(Correspondence: Prof. Francesco Ceccherelli, via Avellino 11, 35142 Padova, Italy Tel:(0039)049-8364121; Fax: (0039) 049-8364121; E mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it ) (Received: February 14, 2014; Accepted with revisions: July 25, 2014)

ABSTRACT:

Acupuncture has been successfully used in myofascial pain syndromes.

However, the number of needles used, i.e. the “dose” of acupuncture stimulation, to obtain the best antinociceptive efficacy, is still a matter of debate. The question was addressed comparing the clinical efficacy of 3 different therapeutic schemes, mainly characterized by different numbers of needles used on 90 patients affected by a painful cervical myofascial syndrome. Patients were divided into 3 groups; the first group of 30 patients was treated with 11 needles, the second group of 30 patients was treated with 5 needles and the third group of 30 patients was treated with 3 needles. Each group underwent eight cycles of somatic acupuncture. In each session and in each group, all needles were stimulated until the pain tolerance threshold was reached;"pain tolerance is the amount of pain a person can handle without breaking down, either physically or emotionally". Pain intensity was evaluated before therapy, immediately after, and at 1 and 3 months follow-up by means of both the McGill Pain Questionnaire and the Visual Analogue Scale (VAS). Pain and the repercussion of pain on the patient’s quality of life (DOPE- Descriptors Of Pain Effects) were also measured using a test we developed, administered at each session. In all groups, needles were inserted superficially, except for the two most painful trigger points that were deeply inserted. All groups, independently from the number of needles used, obtained a good and significant therapeutic effect without clinically relevant differences among groups. For this pathology and patients of this kind, the number of needles, 3 or 5 or 11, seems not to be an important variable in determining the therapeutic effect.

The Beneficial Effect of Electro-acupuncture Given at PC6 (Neiguan-point) by the Increase in Cardiac Transient Outward K+ Current Channel Which Depends on the Gene and Protein Expressions in Artificially Induced Myocardial Ischemia Rats

Professor Ying Wang, MS (1)

Professor Wei Wang, MS (1)

Di Li, MS (1)

Jiquan Li, MS (1)

Professor Jianyu Dai, MD (1)

Yufu Liu, MS (1)

Professor Chunri Li, PhD (1)

Xiaolu Zhang, MS(1)

Professor Peijing Rong, MD (2)

Professor Yiguo Chen, MD (1)

Dept. of Acupuncture and Moxibustion College, Liaoning University of Traditional Chinese Medicine, Shenyang, China (1) Dept. of Institute of Acupuncture, China Academy of Chinese Medicine, Beijing, China (2) Ying Wang and Wei Wang have equally contributed to the work.

(Correspondence: Prof. Yiguo Chen, Tel: +86-024-31207131, E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it and Ying Wang, Tel: +86-024-31207140, E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it ) (Received: August 6, 2014; Accepted with revisions: August 16, 2014)

ABSTRACT:

This study explored the mechanism of electro-acupuncture (EA) at PC6 to improve the heart function by regulating the cardiac transient outward potassium current (=Ito) channel in myocardial ischemia (MI). Kv1.4 is the main component of the slow Ito (Ito.s) channel. Kv4.2 and Kv4.3 are the main components of the fast Ito (Ito.f) channel. KChIP2 is a compound protein of Ito channel. In this experiment, MI was induced by injecting isoproterenol in rats, and the gene and protein expressions of Kv1.4, Kv4.2, Kv4.3 and KChIP2 were lower [The gene and protein decreased (42.8±4.3)% and (37.2±4.7)% respectively.] than the control rats significantly (p < 0.05). After MI induction, the MI rats were divided into three groups, including PC6, LU7 (Lieque-point) and Non-acupoint group which were acupunctured at once a day for 7 days respectively. After EA at PC6 and LU7, the gene and protein expressions showed obvious increased [EA at PC6: the gene and protein increased (50.2±5.4)% and (39.1±5.3)% respectively. EA at LU7: the gene and protein increased (24.6±2.8)% and (22.0±5.5)% respectively] and they are all higher than MI rats significantly (p < 0.05). EA at PC6 and LU7 showed the same variation, and the effects of EA at PC6 and LU7 were better than Non-acupuncture-point (p < 0.05). The effects of EA at PC6 were significantly better [The gene and protein increased (19.7±2.7)% and (14.1±4.4)% respectively] than LU7 (p < 0.05). PC6 is an acupoint of the pericardium meridian, and the pericardium meridian which corresponds to adrenal gland according to Omura Y's research, can affect the heart function directly. LU7 belong to the lung meridian, and the lung plays an important factor in blood circulation according to TCM. So PC6 is more effective than LU7 on heart function regulation. The results suggested that PC6 showed the target effect of meridian specificity on regulating the Kv channel in MI.

The Impact of Acupuncture Treatment on Nitric Oxide (NO) in Migraine Patients

Yasemin Gündüztepe, MSc, PhD Candidate (1), Dept.of Biochemistry

Setenay Mit, MD, PhD (1), Dept.of Biochemistry, Acupuncture Division

Ersel Geçioglu, MD, PhD Candidate, Dept.of Biochemistry, Acupuncture Division

Neslihan Gurbuz, PhD (1), Toxicology Division, Dept. of Biochemistry

Osman Salkacı, MD (2), Dept. of Eye Diseases, Ministry of Health Lufti Kurdar Kartal Hospital

Cinar Severcan, MSc, PhD Candidate (1), Dept. of Biochemistry

Cemal Çevik, MD (1), Dept. of Biochemistry, Acupuncture Division Gazi University Faculty of Medicine (1) Ministry of Health, Training & Research Hospital of Istanbul, Turkey(2)

(Correspondence: Prof. Neslihan Gurbuz Ph.D. ToxicologyDivision, Dept. of Biochemistry, Tel: +90-312-2024161, E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it ) (Received: August 15, 2014 ; Accepted with revisions: September 9, 2014 )

ABSTRACT:

The purpose of this study is to contribute to efforts to understand the pathophysiology of migraine and create an alternative perspective for prophylaxis and treatment of migraine attacks. Acupuncture treatment was applied to the 22 volunteer migraine patients. The acupuncture treatment consists of 5 sessions with 2 sessions per week. Blood samples have been collected before performing acupuncture, after the 1st session and after the 5th session of the acupuncture. And for the control group blood samples were collected only once. In our study the mean serum NO levels in healthy people were (3,58±0,53) while patients with migraine group were (5,55±0,70) respectively. Serum NO levels were 55 % higher in migraine group compared to the control group The NO concentration also decreased after 5th session (30%) (p<0.05). After the 1st sessions NO level decreased 4,86 % in the migraine group. After the 5th session NO level decreased 30,63 % in the migraine group. After the 5th session NO level decreased 27,08% compared to 1st session in the migraine group. After the 1st session of acupuncture treatment, level of NO in migraine group were 4,86 % and it is reduced but statistically not significant. After the 5th acupuncture treatment serum NO levels were reduced to (3,85±0.62) 30,63 % in migraine group and were statistically lower (p<0.05). After the 5th session NO level decreased 27,08% compared to 1st session in the migraine group. In this study, acupuncture treatment is appeared to be effective by lowering the levels of serum NO and acupuncture has cumulative effects. Although there are earlier studies showing the effect of NO in migraine, this has been the 1st study in this field, which shows the effect of acupuncture on NO in migraine patients.