Acupuncture & Electro-Therapeutics Research 40(4) Abstracts

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ACUPUNCTURE & ELECTRO-THERAPEUTICS RES., INT. J., Vol. 40, pp. 297-333, 2015
Copyright ©2015 Cognizant Communication Corp. Printed in the USA.
0360-1293/15 $60.00 + .00
DOI: http://dx.doi.org/10.3727/036012916X14533115160606

Using new non-invasive quick method to detect Borrelia Burgdorferi (B.B.) infection from specific parts of the heart in “seemingly normal” ECGs, and from the ECGs of Atrial Fibrillation (AF), a majority of AF ECGs are found to have: 1) Significant B.B. infection, 2) Markedly increased ANP, 3) Increased Cardiac Troponin I & 4) Markedly reduced Taurine. These 4 factors were mainly localized at infected areas of the SA node area, R-&Latria & pulmonary veins at the L-atrium.

Yoshiaki Omura, M.D., Sc.D., FACA, FICAE, FAAIM, FRSM
Adjunct Prof., Dept. of Family & Community Medicine, New York Medical College; Director of Medical Research, Heart Disease Research Foundation; President, International College of Acupuncture & Electro-Therapeutics; Editor-in-Chief, Acupuncture & Electro-Therapeutics Research, International Journal of Integrated Medicine

Dominic Lu DDS, FICAE
Clinical Prof. of Oral Medicine, University of Pennsylvania; President of American Society for the Advancement of anesthesia and sedation; Visiting Prof. of Holistic Dentistry, International College of Acupuncture & Electrotherapeutics

Marilyn K. Jones DDS, MS, FICAE
Visiting Associate Prof. of Holistic Dentistry, International College of Acupuncture & Electrotherapeutics; Director, Holistic Dental Center of Houston; Former Assistant Prof. of Chemistry, University of Houston, Texas

Abdallah Nihrane, Ph.D., FICAE
Visiting Associate Prof. of Integrative Medicine, International College of Acupuncture & Electro-Therapeutics; Former Assistant Prof., Dept. of Medicine, Mount Sinai Medical School, New York City; Former Visiting Scientist at NIH, Bethesda, MD

Harsha Duvvi MD, MPH, FICAE
Clinical Assistant Prof., Dept. of Family & Community Medicine and Neurologist, New York Medical College; Visiting Associate Prof. of Integrative Medicine, International College of Acupuncture & Electro-Therapeutics

Dario Yapor DDS, FICAE
Visiting assistant professor of Holistic Dentistry, International College of Acupuncture & Electrotherapeutics

Yasuhiro Shimotsuura MD, FICAE
President of Japan Bi-Digital O-Ring Test Association; Visiting Prof. of Integrative Medicine, International College of Acupuncture & Electro-Therapeutics; Executive Director ORT Life Science Research Institute, Kurume City, Japan

Motomu Ohki MS., FICAE (Hon.),
ORT Life Science Research Institute, Kurume City, Japan

(Correspondence: Dr. Omura, 800 Riverside Drive (8-I), New York, N.Y. 10032, Tel: 1 (212) 781-6262; Fax: 1-212-923-2279; E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it )

(Received October 28, 2015; Accepted with revision: December 15, 2015)

Lyme disease is found in a majority of people we tested. Once Borrelia Burgdorferi (B.B.) spirochete enters human body, it not only causes pain by infecting joints, but it also often enters the brain and the heart. Infection of brain can be quickly detected from the pupil and infection of the heart by ECGs noninvasively. By evaluating recorded ECGs of atrial fibrillation (AF), using U.S. patented non-invasive highly sensitive electromagnetic field (EMF) resonance phenomenon between 2 identical molecules or between a molecule and its antibody, we examined 25 different AF patients’ ECGs and found the majority of them suffer from various degrees of B.B. spirochete infection in SA node areas, also in the right & left atria, and pulmonary vein near and around its junction at left atrium & lesser degrees of infection at the AV node & His Bundle. When B.B. infection reaches over 224~600ng or higher at these areas, AF often appears in the majority of all AF analyzed. In order to develop AF, the 4 abnormal factors must be present simultaneously: 1) B.B. infection must be increased to 224~600ng or higher, 2) Atrial Natriuretic Peptide (ANP) must be markedly reduced from normal value of less than 4ng to over 100~400ng, 3) A significant increase of Cardiac Troponin I from normal value of less than 3ng to over 12ng and 4) Taurine must also be markedly reduced from normal value of 4~6ng to 0.25ng. These 4 changes were mainly found only at infected sites of the SA node area, both atria and between the end of the T wave & the beginning of the SA node area, which corresponds to U waves at recorded ECG. Origin of the U wave is mainly due to abnormal electrical potential of pulmonary vein at L-atrium. If all 4 factors do not occur at the infection site, no AF will develop. In seemingly normal ECGs, if using this method, one can detect invisible B.B. infection in early stages. Long before AF appears, AF can be prevented by improved treatment with Amoxicillin 500ng 3 times/day + Taurine 175mg x3 times/day, with or without EPA 180mg & DHA 120mg, to avoid serious current limitations in the use of Doxycycline 100mg 2 times/day, for 4 weeks.

Key Words: Atrial Fibrillation; Lyme disease; Lyme carditis; Borrelia Burgdorferi (B.B.) spirochete; Atrial Natriuretic Peptide (ANP); Cardiac Troponin I; Taurine; Vitamin D3; SA Node; Right & Left atrium; AV Node; Acupuncture at painful joint; Amoxicillin; Doxycycline


ACUPUNCTURE & ELECTRO-THERAPEUTICS RES., INT. J., Vol. 40, pp. 335-353, 2015
Copyright ©2015 Cognizant Communication Corp. Printed in the USA.
0360-1293/15 $60.00 + .00
DOI: http://dx.doi.org/10.3727/036012916X14533115160642

The Beneficial Effects of Electro-acupuncture at PC6 (Neiguan-point) of Gene and Protein Expressions of Classical Inward-rectifier Potassium Channels in Myocardial Ischemic Rats

Professor Ying Wang, MS(1)
Xiao-lu Zhang, MS(1)

Professor Wei Wang, MS(1)

Di Li, MS(1)

Professor Jian-yu Dai, MD(1)

Professor Chun-ri Li, PhD(1)

Ji-quan Li, MS(1)

Professor Yi-guo Chen, MD(1)

Professpor Pei-jing Rong, MD(2)


(1)Dept. of Acupuncture and Moxibustion Colleage, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China
(2)Dept. of Institute of Acupuncture, China Academy of Chinese Medicine, Beijing, China

(Correspondence to: Prof. Yi-guo Chen, Tel:+86-024-31207131, E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it and Ying Wang, Tel:+86-024-31207140, E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it )

(Received: Nov. 27, 2015; Accepted with revisions: Dec. 27, 2015)

This study is aim to investigate the effect of electro-acupuncture at PC6 (Neiguan-point) on the gene and protein expressions of classical inward-rectifier potassium channels (Kir) in myocardial ischemia (MI) rats induced by isoproterenol (ISO). With ten for each one, 50 rats were divided into 5 groups which were control group, MI group, PC6 group, LU7 (Lieque-point) group and non-acupoint group. The control group was injected normal saline solution (85mg/kg), the other groups were injected ISO (85mg/kg). All the rats were injected once daily for two days and recorded electrocardiograms (ECGs) after every injection. Electro-acupuncture (EA) was operated at PC6, LU7 and non-acupointrespectively in the rats of PC6 group, LU7 group and non-acupoint group after twice injections. EA was performed to these three groups with disperse-dense wave (4-20Hz), pulse amplitude of 14V, 20mins a day remaining 7 days. The gene and protein expressions of Kir2.1, Kir2.2 and Kir2.3 were analyzed by Western Immunoblotting Technology (Western Blot) and Real-time Fluorescence Quantitative Polymerase Chain Reaction (RT-PCR). But it is regrettable that we did not detect meaningful gene and protein expressions Kir2.3, and the expressions of Kir2.1 and Kir2.2 in MI induced groups were lower [The gene and protein decreased 39.4±27.3% and 38.7±17.1% respectively.] than control group (P<0.05). Compared with MI group, the results of PC6 group and LU7 group increased [PC6 group: the gene and protein increased 42.9±25.0% and 42.2±10.0% respectively. LU7 group: the gene and protein increased 23.8±50.1% and 21.1±32.5% respectively.] obviously (P<0.05) after EA, furthermore the expressions of PC6 group were higher [The gene and protein increased 15.4±16.7% and 17.3±60% respectively.] than LU7 group (P<0.05). The results show that PC6 has a better positive effect than LU7 on MI rats, and the mechanism is probably that EA at PC6 can significantly increase the gene and protein expressions of Kir2.1 and Kir2.2.

Keywords: Myocardial ischemia; Electro-acupuncture; Classical inward-rectifier potassium channels; Gene; Protein; PC6 (Neiguan-point); LU7 (Lieque-point); Electrocardiograms (ECGs)


ACUPUNCTURE & ELECTRO-THERAPEUTICS RES., INT. J., Vol. 40, pp. 355-369, 2015
Copyright ©2015 Cognizant Communication Corp. Printed in the USA.
0360-1293/15 $60.00 + .00
DOI: http://dx.doi.org/10.3727/036012916X14533115160688

Electro-Acupuncture at GV.4 Improves Functional Recovery in paralyzed rats after a Traumatic Spinal Cord Injury.

Oswaldo JuarezBecerril, M. D. Professor1
Hermelinda SalgadoCeballos, Ph. D.Researcher Professor
2,3
Candy AnguianoSolis, M. D. Candidate
1
Belen AlvaradoSanchez M. D. Professor
1,5
Monica Elba Lopez Hernandez, Researcher Fellow
1
Araceli DiazRuiz, Ph. D. Researcher
4
Sergio TorresCastillo, Ph. D. Researcher Professor
1,3*

1Licenciatura en Acupuntura Humana Rehabilitatoria, Universidad Estatal del Valle de Ecatepec, Estado de México, México
2Unidad de Investigación Médica en Enfermedades Neurológicas, CMN, SXXI, IMSS, Ciudad de México, México
3Proyecto Camina A. C. Ciudad de México, México
4Instituto Nacional de Neurología y Neurocirugía, Ciudad de México, México
5Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México

(Correspondence: Sergio Torres Castillo, Ph.D., Calle Chidal No. 11, Col. Cd. Azteca 3ª. Sección, C. P. 55120, Ecatepec, Estado de México, Tel: 55 + (50 01 14 00) Ext: 107, e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it )

(Received February 12, 2014; Accepted with Revisions: July 31, 2015.)

In the present study, the effect of electro-acupuncture (EA) on the oxidative stress, the spinal cord tissue preservation and the recovery of motor function was evaluated after a traumatic spinal cord injury (TSCI). Long Evans rats were randomized into five groups: 1. Sham; 2. TSCI without treatment; 3. TSCI + EA (acupoint GV.4); 4. TSCI + EA (acupoint GV.26) and 5.TSCI + EA (GV.4 + GV.26). The EA was performed with an Electro-Acupunctoscope, AWQ-104L Digital, wave dense-dispersed, current intensity 2.5mA and frequency 2-100Hz for 30 minutes. The biochemical results showed a significant increase in the hydroxyl radical concentration in group 2 (3.1±1.4nmol) compared with groups 1 (1.8±0.5nmol) and 4 (2.4±1.1nmol) (p<0.05), whereas in group 4 (4.8±1.8nmol), there was a significant increase in lipid peroxidation when compared with group 1 (1.7±0.5nmol) (p<0.05). The BBB motor function score in the paralyzed hind limbs (normal BBB=21points) was greater in groups 3 (15.2 points) and 5 (13.5 points) in comparison with groups 2 (11.4 points) and 4 (9.3 points) (p<0.05). The quantity of preserved spinal cord tissue was greater in group 3 (6582.7±20μm2) than in groups 2 (5262.4±20μm2), 4 (3995.6±26μm2) and 5 (4266.7±22μm2). Although EA in GV.26 decreases hydroxyl radical concentration (50%), it significantly increases lipid peroxidation (45%), while stimulation of GV.4 decreases oxidative stress (15%), preserves spinal cord tissue (25%) and improves recovery of motor function in the hind limbs of rats with paralysis (18.1%) compared with untreated group. These findings suggest that EA in GV.4 may be a therapeutic alternative on TSCI.

Key words: Traumatic Spinal Cord Injury; Oxidative Stress; Electroacupuncture; Acupoint GV.4; AcupointGV.26;Lipid peroxidation; Motor function recovery.